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KMID : 0043320140370101271
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Archives of Pharmacal Research
2014 Volume.37 No. 10 p.1271 ~ p.1279
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Preparative isolation of aldose reductase inhibitory compounds from Nardostachys chinensis by elution?extrusion counter-current chromatography
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Paek Ji-Hun
Lim Soon-Sung
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Abstract
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A method combining enzyme assay-guided high-performance liquid chromatography (HPLC) micro-fractionation and elution?extrusion counter-current chromatography (EECCC) was developed to screen and separate aldose reductase (AR) inhibitory activities from those of the ethyl acetate (EtOAc) fraction of Nardostachys chinensis. Under the target-guidance of HPLC micro-fractionation, two phenolic compounds, three caffeoylquinic acid derivatives and two sesquiterpene were isolated by high-speed countercurrent chromatography (HSCCC) using elution modes of extrusion?elution. A one?step HSCCC isolation method was developed, which included a solvent system of n?hexane?EtOAc?methanol?water at a ratio of 2:8:3:7 (v/v/v/v). The chemical structures of the isolated compounds were determined using 1H? and 13C?nuclear magnetic resonance spectrometry. The compounds inhibiting AR in the EtOAc fraction of 70 % ethanol extracts of N. chinensis were identified as chlorogenic acid (2) and 1,5-di-O-caffeoylquinic acid (6). Our results indicate that the combined method of HPLC micro-fractionation and EECCC is fast, efficient, and reproducible for systematically isolating AR inhibitory compounds from complex natural products.
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KEYWORD
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Aldose reductase, Nardostachys chinensis, Micro-fractionation, Elution-extrusion counter-current chromatography, 1, 5-Di-O-caffeoylquinic acid
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